Medicine and disease in the fly's wing
This research was conducted to identify the disease and the cure in "modern flies" the Messenger of Allah, peace be upon him. To respond to skeptics in this talk. 9 species were isolated from bacteria positive and Gram-negative, as well as two types of yeast (fungi). These organisms were isolated from the right and the left wing of the four types of insects, namely: Fly Home Musca domestica, the stable fly false Muscina stabulans, sand fly Phlebotomus papatasi, and household mosquito Culex pipiens. These insects have been compiled from different environments in the governorates (Giza, Cairo and South Sinai) and that by air network or electrical Bchwet mosquitoes. Was isolated using microorganisms among six different food and non-optional optional so as to isolate the largest number of micro-organisms. The numbers of bacteria isolated from recorded among yeast extract agar nutrient and blood Trepettoz the largest number among all the species isolated study proved that the bacteria Bacillus circulans- (T 88) were stronger bacterial species in the secretion of the most effective metabolic article. I have been to isolate this dangerous kind of the right wing for each of the household fly and the stable fly false. Physical and chemical characteristics of the material free of impurities metabolic shown to be of the nature of the aromatic compound was determined the chemical formula of the compound which is C30H37N4SO9. The study activity against microbial to this article on many kinds of microbes isolated from flies and other microbes from outside the flies were most influential boat is bacteria Gram-positive pathogenic and was less sufficient concentration to cause inhibition of harmful bacteria growth process is 5 ug / ml. Provided that the research in the field of microbes associated with insects is one of the exciting studies, where the relationship between microbes and insects may be carried only Phoresy or Tkavlih Mtaabshh relationship or a relationship. The role of the microbes associated with insects in the transmission of the disease or spoiling food. Has been studied by many scientists Taylor (1935), Grecnberg (1973), Alcanos and Frishman (1980), Mcoay et al (1982). The other scientists discussed the relationship of cohabitation between microbes and various types of insects such as Breznak (1982), Fouda (1984), Hassan et al (1996, 1998a, 1980b, 2000). Check Ghanem et al (1986) types of bacteria located on the outer surface and in the members of the three different types of insects. It is not obtained any Arab or foreign reference studied the different types of microbes associated with any kind of wings flies. The research is the first research offers in this area at the international level and local level. Aims current research is to isolate the different types of microbes located on the wings of three types of flies are: Fly Home, fly the stable false, the sand fly in addition to the mosquito, and that in order to reach scientific facts and identify the disease and medicine in the wings of flies true to modern Prophet Muhammad him. It is the right of the conversations. As it narrated by many narrators. Hadith: and you bond: Male (Dr / Khalil Ibrahim peace Khater, support modern and board in his book (the injury in an interview with the fly). And also quoted Dr / Karem Ghoneim support modern and board in writing scientific references in the hadith. I have narrated talk many narrators such as: narrated by Bukhari in his Sahih, Ibn Majah in his Sunan Abu Huraira say Messenger Allahsaly God be upon him: (If flies occurred in the syrup of you Vlagamsh then to be dispelled, the in one of its wings disease At the other healing). mentioning Bazaar, as well as Tabrizi in (Mishkat LEDs), and Ibn Hajar in (summarized Habeer). narrated by Bukhari in his Sahih, Ibn Majah in his Sunan and Ahmad in his Musnad from Abu Hurayrah that he said: The Messenger Allahsaly God be upon him: (If flies occurred in the syrup of you Vlagamsh - all - and then to be dispelled, the in One of its wings disease At the other healing). mentioning Bazaar, as well as Tabrizi in (LEDs) niche, and Ibn Hajar in (summarized Habeer). narrated by Bukhari in his Sahih from Abu Huraira that the Messenger of Allahsaly be upon Slmqal: (if flies occurred in a vase of you Vamgulwh , in one of the wings disease At the other healing). Agamsoh and Amgulwh means, as stated in the end to the son of the ether. Ibn Majah in his Sunan from Abu Sa'eed that the Messenger of Allah be upon him and Allahsaly Slmqal: (one wing flies cm and the other healing, if signed in the food, Vamgulwh, it offers poison and delay healing). Women and narrated by Ibn Majah in Snnehma Khudri that the Messenger of Allah be upon him and Allahsaly Slmqal: (The one in the wings of flies cm and the other healing, if signed in the food, Vamgulwh, it offers poison and delay healing). This, has cited the owner (the bottle lamp) (1) newly brief for Women in (Sunan Minor) for the Amr ibn Yahya bin Saeed Al-Qattan Ibn Abi wolf, word flies :( If any of you in a vase signed Vlamqlh). And Ibn Abi wolf happened Ahmad in his Musnad, too, he said: Tell us Yahya, narrated by Ibn Abi wolf said: Tell me about Saeed Bin Khaled Abu Salamah, from Abu Sa'eed, Allenbaisaly God for him and Slmqal flies :( If any of you signed in food Vamgulwh) . And narrated by Ahmad in his Musnad, also, for and Kia for Ibrahim bin Fadl, Said ibn Abi Said Abu Hurayrah said: The Messenger of Allahsaly God it said him: (If flies occurred in the food one of you or drink Vlagamsh then get it out, the in one of its wings disease in The other cure, and it offers a disease). And narrated by Ahmad in his Musnad, also, for Younis, from Leith, for Mohammed, for Qaqaa, from Abu Salih, from Abu Huraira, the Messenger Allahsaly be upon Selmana said: (The flies in one of its wings disease At the other healing, if signed in a vase Vlagamsh one of you, it is him who fears the disease, then get it out). Roy recaps in his Sunan from Suleiman ibn Harb, Hammad ibn Salamah, for Thamama bin Abdullah ibn Anas, from Abu Hurayrah Allenbaisaly God be upon him said: (If the flies took place in a pot of you Vlagamsh, is in one of its wings disease At the other healing) . Al-Bukhaari in his Saheeh from Qutaiba, Ismail bin Jafar, Ben Muslim sire threshold structures Tim, for Obaid bin nostalgia sire built Zurayk, from Abu Huraira that the Messenger of Allah, may Allah bless him and Slmqal: (if flies occurred in a vase of you Vlagamsh whole then posed , in one of the wings and in the other healing disease). Track author of the book (injury) (2) support modern fly in full door of his book, and make the first chapter about ways in which this conversation, and the second chapter on the study of the grounds this talk. The modern ways handled in five, are: Abu Huraira ways, the hadeeth of Abu Sa'eed al ways, Anas Ibn Malik ways, and methods of modern Ali bin Abi Talib .. The Abu Huraira has Bukhari of the two roads, and narrated by both Abu Dawood and Ibn Majah than one way, and directed by Ahmad ibn Hanbal than ten ways, and narrated recaps of the two roads, and narrated by Bayhaqi of three ways, and narrated by each of Ibn Khuzaymah and Ibn son Jaarood than one way, and narrated by Eltahawy of six ways, and narrated by Baghawi of three ways, and narrated Ibn al-Sakan and Ibn Abd al-Barr, etc ... Thus, even the owner of the book counted thirty-four way in terms of the whole, and forty-two way in terms of detail, and the narrators talk proved all these roads. Image of glass to fly home and then eat the hadeeth of Abu Sa'eed al-ways (to fly), was narrated by Ahmad ibn Hanbal from two roads, and all of the Women's and Ibn Majah and Ibn Abi Dawood Tayaalisi and Bayhaqi and Ibn Qutaiba and Abu Obeid, Ibn Abd al-Barr and Baghawi and Abdullah bin Humaid, from one way, and narrated by Eltahawy of two roads, as well as Tabaraani Abu second to none and the ruling .. etc. So did the author of the book for the modern methods when Anas bin Malik and when Ali bin Abi Talib. The last novel - the generosity of God and face - Vokhrjha the carpenter's son, but pronounced: (in flies: a disease wings, and in the other healing, if signed in the pot, Vorspoh, goes initially cured). And author of the book dealing with the study of the modern grounds of different ways, and in this study proved beyond a reasonable doubt the validity of this talk, talk, then it is corrected, it is of the highest degrees of health. Then concluded by saying: that this news (ie: talk flies) may abounded his ways so that increased fifty path, as is the decree in the "trees of the novel," each of the Abu Huraira and dad happy and Anas - may Allah be pleased with them all. Also, these methods have been received isnaads correct and its men are trustworthy .. Thus it is clear that this talk is true in terms of the novel and Sindh, and the rule of imams by health say is true sound, nothing wrong, and that it violated and denied - in terms of the novel - he has proof, and crying to the moon, and that I have, it has been narrated by imams, the trustworthy, thank God. And the Hadith did not call anyone to catch flies and place it forcibly into the vessel, was encouraged to leave vessels open, not to neglect cleanliness of homes and streets, and does not conflict with protection from the dangers of the proliferation of flies in any way, and did not compel the impact of flies in his vessel and nauseate it on eating it: (God does not burden any soul beyond its scope). This talk does not prevent anyone from existing on people's health, not from doctors to cope with the flies and the resistance of different means. It can not come to mind (the mind of the religious scholars or others) that this calls for the establishment of the modern farmer flies. But if taking another talk, "one of his wings in the disease and in the other healing." We find our noble invites us to search and go to study flies to see what is the disease that exists on one of the wings of flies, and also to the knowledge of medicine or healing who's on the other wing in order to reach Muslim Scholars of medicines that address diseases transmitted by flies. The study we find that it includes modern physics, medicine, pharmacology, microbiology, insects as well as religion and language sciences. Ways and means used 1 - flies collection: The collection of two types of flies is sucking blood are: Fly Home, and the stable fly false. It was collected sand flies that suck human blood, animal and also has been collecting household mosquito that feeds on human blood and animal. These insects were collected from the governorates of Cairo, Giza, and South Sinai. Insects have been transferred in a sterile tubes to the laboratory for autopsy and isolate microorganisms from them. 2 - Anatomy of flies: were dissected flies to separate each of the right wing and the left wing for each fly by instruments Anatomy accurate and sterile for a number 20 insect of every kind, was then put all of the right wing and the left wing separately for each fly in physiological sterile solution ( Hlamin 0.9). 3 - isolate microorganisms: 50 Maikarolter were taken from each sample were placed on farms bacterial following: 1- Nutrient agar emended with 1% yeast extract. 2- Nutrient agar emended with 5% sheep blood 3- MaConkey's agar 4- Starch nitrate agar 5- Tryptose blood agar 6- Staphylococcus media have been previous bacterial communities in the incubation temperature of 30 ° under anaerobic conditions mode. Was counting bacteria (consisting of the colony units (CFU after 48 hours after it has been defined bacteria even species level. In accordance with the reference taxonomic bacteria for each of the (1944) Holt et al. ,, (2004) Honda et al. 4 - exclusive analysis of activity against microbial : This was done by paper analytical tablets to study activity against microbial for different types of bacteria against each 0.5 - the process of fermentation: The study stronger Bacterial Farms effect have been isolated from the previous method against each other in order to get the most powerful type of bacteria are effective against other types. 6 - extraction and purification of the compound metabolic: was selected organic solvent appropriate using Bioautographic technique and that at different pH values. then purifying the extract by Thin layer and column chromatography. was used as the first device a second time to confirm the purity of the compound metabolic analysis of spectral activity. was Spectroscopy study pure using the active compound Ultra-violet (UV) and a Spectrophotometer, as well as infrared (IR) was obtained Mass spectral Data by Hp MS mudel device 59 887 - Evaluation of less inhibitor bacteria concentration (MIC): This was done using the method of Agar Diffusion Method to get the lowest inhibitor concentration of the compound active against different types of bacteria, yeast and fungi isolated from flies and flies out. Results and discussion resulted in the examination of each of the two wings fly home, and the stable fly false, sand flies and mosquitoes for the presence of heavy diversity and many of the existing types of micro-organisms on them. I have recorded the highest numerical density and multiplicity of types Kabtrella and fungi on the wings of the stable fly home and fly false as shown in Table 1. Gram-positive bacteria were present numerical density greater than in Gram-negative bacteria. Right wing higher numerical density of Gram-positive bacteria in all sorts of flies record. As pointed out by Hassan, et al (1998a) that flies microbial diversity reflects the environment in which the flies live. This means that for each environment of certain types of micro-organisms is different from any other environment. It was observed that Gram-positive bacteria has recorded the highest numerical density of Gram-negative bacteria. This illustrates the ability of living in difficult circumstances, as they bear the heat, the cold, the impact of chemicals and radiation. Record Sex Bacillus50% of all species of bacteria isolated, especially Gram-positive bacteria. Characterized isolates of Gram-negative bacteria Table (2) that have medicinal importance especially in terms of its ability to cause many diseases. I have been to isolate three races of this bacteria are: Salmonella, Erwina and Pseudomonas. The latter affects human and animal sex and also the plant. And surprisingly it has been isolated this race from the left wing to the stable fly over the center of false food MacConkey. And this has been isolated by Gender Ahmed et al (1995) on the surface of each of the outer fly home and fly the stable and exempted livestock and current researcher has participated in this research. Table (2) isolate strains of yeast, where he found that its oval shape, and reproduce by budding. I have observed that one of them produces many sugars on the cell material. We have isolated a former researcher at the types of fungi called Search Empusa muscae have the ability to secrete some antibiotics, which can kill many types of negative and Gram-positive bacteria. I have managed all of the "Arnstein" and "cook" The English in 1947, and "Roljus" Swiss in 1950, to isolate antimatter vitality called "Javasan" of the mushroom of the same species that we have mentioned and that live in the fly. It was also in this research as shown in Table (2) isolate some types Alaktinomaysts which also have the ability to secrete some antibiotics. Table (3) anti-bacterial effect of various types that have been isolated. It was noted that some types of bacteria such as Salmonella and Erwina and Lactobacillus gasseri have little effect against other types of bacteria. While there are five types of bacteria have a high efficiency in the elimination of other types of bacteria are: B. circulans, L. animalis, B. subtilis, P. aeruginosa and S. aureus. I have been brewing this bacteria and analyzed to determine their impact against each (Table 4). Table (4) and form (1) that the most common types of bacteria effectiveness is B.Circulans The strongest object of every species and I have observed the presence of this type of bacteria on the right to fly wing which bear high temperatures, radiation, chemical effect and cold. After it has been prove that B.Circulans is the most powerful types of bacteria located and isolated from the right wing flies, it has been subjected to isolate them effective article. It has been prepared in the form of powder, it does not have a particular form and color yellowish white. It was noted that the degree of melting 185 ° m. And soluble in alcohol and chloroform as they dissolve in water. But they do not dissolve in petroleum ether. The active substance analyzed using Thin layer chromatography analysis has resulted in the presence of 5 amino acids. The analysis also pointed out the existence of a high percentage of nitrogen content in the article. The article analyzed using Mass spectra of pure material was determined the chemical formula of the compound which is C30H37N4SO9 (Figure 2). Aodhjdol (5) Bioautography Article effective metabolic interaction with various solvents, it was noted that it is moving as a single. Figure (3) analysis of the active ingredient by absorption spectroscopy UV UV. As it is shown in the form of (4) analysis of material using infrared IR. It was also used metabolize 1H-NMR analysis of this article and find out installed. Previous analyzes of all turned out to be the active ingredient of anti-vital her installation aromatic. The similarity in the nature of her other vehicles toroidal nature as stated in some research (Zhang et al 1999). A table showing (6) less sufficient concentration to inhibit the growth of microorganisms Minimum Inhibatory Concentration (MIC). It has turned out that the lower concentration of the active ingredient was isolated a deadly effect against many types of negative or Gram-positive bacteria, yeast against against filamentous fungi. Form shows (6) gave me enough time to kill the bacteria and found that less concentration is 5 mg / ml is enough to kill many kinds of bacteria. It has turned out to be the active ingredient isolated its active effect in this area, since the preparation of the bacteria has been reduced to about 0.01% in a short time. It has been more affected by the types of bacteria are: B. subtilis and S. aureus, two of the most common types of bacteria pathogenic to humans and cause many diseases such as eye infections, abscess or boils, impetigo (a skin disease), cystitis, inflammation of the stomach and colon, inflammation bone, genitourinary system injury, central nervous system and the corruption of foods and other .... conclusion (the face of the scientific miracles) is evident from the above results and a high numerical density of many types of bacteria on the three wings of the types of flies, while I prepare bacteria and types on the wings Mosquito. As it turns out that the more aggressive types of bacteria is a type B. circulans which secretes anti-material vital to many types of bacteria and other, whether negative or Gram-positive. I have observed the presence of this bacteria on the high density of the right wing of the flies. The presence of types of fungi that also secrete anti materials vital to many types of bacteria was also observed. As it turns out the bacteria B. circulans's ability to kill other types of bacteria in a very short time. It is the bacteria that transmit many diseases to humans and that have been mentioned. Image-bacterial B. circulans If we go back to talk Messenger of Allah, peace be upon him Abu Huraira text: "If the flies took place in a pot of you Vlagamsh then posed to the disease in one of the wings and in the other healing." Fa, we find that character in "Vlagamsh" According to speed, while "and then" stating lax and slow. So the Prophet Muhammad, peace be upon him by drowning the flies ordered quickly because it relates to on the surface of the liquid to the presence of surface tension and the word then after dipping give a chance for beneficial bacteria and fungi to produce antioxidants for vital and medicine or healing) in order to eliminate the harmful bacteria (disease). It has been shown that even if a person ate or drank from the vessel, the active ingredient remain active in the human intestine because these bacteria in the case of its livelihood in the intestines of the host. They also bear the high temperatures, radiation effect, the effect of chemicals and cold any that flies even if it fell into the bowl with food or hot or cold drink, the beneficial bacteria (drug) remain active and secrete the active substance lethal to other types of microbes less concentration of a 5 mg / ml. That is, 5 grams of material sufficient to sterilize 1,000 liters of milk or any liquid or food. Perhaps the greatness of Prophet Muhammad, peace be upon him in the matter by drowning the flies evident in mechanical secretion of active substance (drug) as the secretion of the types of beneficial bacteria and fungi of these materials are not taken only in the presence of the center, which is food or drink inside the pot. It allows this because the center meet all of the disease, medicine and face-to-face unhindered and are docking at which time the eradication of harmful organisms Useful objects. It was found that antimatter and vitality that kill negative or Gram-positive bacteria Ataathrr of fungal cells only if and when the liquid is absorbed, it by osmotic pressure property swell and then explode and release their contents, which are considered such as grenades and the elimination of harmful bacteria. It was noted that these bombs thrown to a distance of 2 mm liquid inside which is a great distance relative to the size of micro-organisms. In many research earlier carried out by the current researcher with others, most of pathogenic bacteria isolated from the outer surface of the flies, especially of the legs and abdomen, such as bacteria: winery anthrax, typhoid, Albaratifued, dysentery, eye diseases, respiratory system, digestive system, nervous system, Rev. II. Egypt.